Bacillus subtilis Expression Systems: With its ability to secrete proteins directly into culture media, amenability to medium- and large-scale fermentation, no significant bias in codon usage, and designation by the U.S. FDA as an organism that is Generally Regarded As Safe (GRAS), it‘s not surprising that the majority of industrially-produced enzymes are expressed in Bacillus subtilis. Features: Tightly controlled gene expression / suitable for metabolic engineering • Avoid expensive inducers (e.g., IPTG) • Strain with reduced protease activity available • Endotoxin-free • Prevent protein aggregation • Easy transformation • E. coli / B. subtilis shuttle vectors with different tags • Different constitutive expression systems and vectors for high-level extracellular protein production
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