Bacillus subtilis Expression Systems: With its ability to secrete proteins directly into culture media, amenability to medium- and large-scale fermentation, no significant bias in codon usage, and designation by the U.S. FDA as an organism that is Generally Regarded As Safe (GRAS), it is not surprising that the majority of industrially-produced enzymes are expressed in Bacillus subtilis. With the B. subtilis Constitutive Expression System MoBiTec offers an easy-to-handle tool for high yield protein production with B. subtilis. Features: • Pgrac vectors are structurally and segregationally stable • Inducer-free high-level production of the target protein in B. subtilis • Convenient cloning due to B. subtilis / E. coli shuttle vectors • Strong repression of the target gene expression in E. coli lacIq strains • For subsequent purification of the protein of interest, the Pgrac constitutive expression vectors encode for 8xHis- or Strep tag II • Control vector available (expressing ß-galactosidase)
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