pBacTag Tagging and Integration Vectors - Directed Functional Studies of Bacillus subtilis Genes by Chromosomal Integration. The pBacTag Tagging Vectors enable the directed functional analysis of genes. Tagging or inactivation of target genes is achieved by chromosomal integration of a pBacTag Vector via homologous recombination. Features: Directed functional studies of B. subtilis genes by chromosomal integration • The gene of interest can be selectively inactivated within the chromosome allowing phenotypic studies of the resulting mutant • Alternatively, the gene of interest can be expressed chromosomally as translational fusion protein with an N-terminal epitope or localization tag • After inactivation or tagging, genes localized directly downstream of the gene of interest can be controlled by an IPTG inducible promoter to ensure their expression • Epitope tags (FLAG, cMyc, and HA) can be used for selective purification of fusion proteins and for their detection by commercially available antibodies •Localization tags (GFP+, CFP and YFP) are helpful for localization studies of target proteins
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