This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[235-1]
Molekulargewicht:
70kDa & 80kDa
Isotyp:
IgG1
NCBI:
Not Known
UniProt:
Not Known
Formulierung:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood),Immunofluorescence (1:50-1:100 for 30 minutes at RT),Optimal dilution for a specific application should be determined.
Flow Cytometric analysis of Human Nuclear Antigen on HeLa cells. Black: cells alone, Green: Iso
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