This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000 - 1:25,000, Gel Shift: 0.5 µL - 1.0 µL, IHC: 1:200, IF Microscopy: 1:200, WB: 1:2,000
Anwendungsbeschreibung:
Anti-NFKB p65 antibody has been tested in ELISA, ICC, WB, and IF. NFkB gel shift assays are assembled in 20µl reactions containing 0.28 pmoles NFkB oligo in 10mM Tris (pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10% glycerol. Some procedures specify th
Rockland Immunochemicals Rabbit anti-p65
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