DAPI Fluoromount-G

Produktübersicht

• Artikelname: DAPI Fluoromount-G
• Artikelnummer: SBA-0100-20
• Hersteller Artikelnummer: 0100-20
• Alternativnummer: SBA-0100-20
• Hersteller: SouthernBiotech
• Kategorie: Biochemikalien
• Applikation: Apop, FISH, ICC, IHC, IHC-Fr, IHC-P, PLA

Produktbeschreibung

DAPI Fluoromount-G is a water-soluble, 4,6-diamidino-2-phenylindole (DAPI) containing compound for slides mounted after a staining procedure having an aqueous final step. It is suitable for microscopy applications utilizing frozen and paraffin sections,

Zusatzinformationen

• Description: DAPI Fluoromount-G® is a water-soluble, 4',6-diamidino-2-phenylindole (DAPI) containing compound recommended for slides mounted after a staining procedure having an aqueous final step. When bound to DNA, DAPI fluoresces and is typically used as a nuclear/chromosomal counterstain. Since DAPI Fluoromount-G® is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened. This mounting medium also provides a semi-permanent seal for storage of slide preparations.
• Buffer Formulation: Phosphate buffered saline containing glycerol, < 0.1% sodium azide, and proprietary ingredients
• Volume: 20.0 mL
• Storage & Handling: Store at 22-25°C
  Please refer to product specific SDS
• Applications: Immunohistochemistry-Frozen Sections 1-9
  Immunohistochemistry-Paraffin Sections 7,10-14
  Immunocytochemistry 15-44
  In situ Hybridization 3,36,43,45-47
  TUNEL 7,11,35,48
  Proximity Ligation Assay 49,50
  Migration Assays 21,23,31,33
  Apoptosis 38
• Recommended Dilutions: Please refer to product specific

Bilder

HCC827 cells were stained with anti-E-cadherin followed by a secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Narita M, Shimura E, Nagasawa A, Aiuchi T, Suda Y, Hamada Y, et al. Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c. PLoS One. 2017;12(2):e0172115. Figure 4(e)

SDV infected rainbow trout satellite cells were stained with anti-E2 followed by an AF488 secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Biacchesi S, Jouvion G, Mérour E, Boukadiri A, Desdouits M, Ozden S, et al. Rainbow trout (Oncorhynchus mykiss) muscle satellite cells are targets of salmonid alphavirus infection. Vet Res. 2016;47:9. Figure 4(a)

Human lung mast cells were stained with anti-TRPC6 followed by an AF488 conjugated secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Wajdner HE, Farrington J, Barnard C, Peachell PT, Schnackenberg CG, Marino JP Jr, et al. Orai and TRPC channel characterization in FcεRI-mediated calcium signaling and mediator secretion in human mast cells. Physiol Rep. 2017;5:e13166. Figure 2(c)

Mutated FSHR transfected COS-7 cells were stained with anti-FSH-receptor followed by an AF555 conjugated secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Hugon-Rodin J, Sonigo C, Gompel A, Dodé C, Grynberg M, Binart N, et al. First mutation in the FSHR cytoplasmic tail identified in a non-pregnant woman with spontaneous ovarian hyperstimulation syndrome. BMC Med Genet. 2017;18:44. Figure 3(d)

Notch1 transfected HEK293T cells were stained with anti-O-GlcNAc and anti-Notch1 followed by AF555 and DyLight 488™ conjugated secondary antibodies and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, et al. O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals. Elife. 2017;6:e24419. Figure 2(d) DyLight™ is a trademark of Thermo Fisher Scientific, Inc.

Frozen section of Pvalb-IRES-CRE mouse CA1 hippocampus injected with AAV-hM4Di-mCherry was stained with anti-parvalbumin followed by a secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Ognjanovski N, Schaeffer S, Wu J, Mofakham S, Maruyama D, Zochowski M, et al. Parvalbumin-expressing interneurons coordinate hippocampal network dynamics required for memory consolidation. Nat Commun. 2017;8:15039. Figure 2(a)

Frozen E15.5 embryonic mouse brain section was stained with anti-HMGB2 followed by a Rhodamine Red ™-X conjugated secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Abraham AB, Bronstein R, Chen EI, Koller A, Ronfani L, Maletic-Savatic M, et al. Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells. Proteome Sci. 2013;11:18. Figure 2(e) Rhodamine Red™ is a trademark of Thermo Fisher Scientific, Inc.

Human neutrophils grown in serum-free media were stained with anti-neutrophil elastase followed by an AF555 conjugated secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Kamoshida G, Kikuchi-Ueda T, Nishida S, Tansho-Nagakawa S, Kikuchi H, Ubagai T, et al. Spontaneous formation of neutrophil extracellular traps in serum-free culture conditions. FEBS Open Bio. 2017;7:877-86. Figure 1(a)

Human neutrophils grown in serum-free media and EDTA were mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Kamoshida G, Kikuchi-Ueda T, Nishida S, Tansho-Nagakawa S, Kikuchi H, Ubagai T, et al. Spontaneous formation of neutrophil extracellular traps in serum-free culture conditions. FEBS Open Bio. 2017;7:877-86. Figure 6(a)

Paraffin embedded mouse left ventricular section post myocardial infarction and intravenous injection with DiI-labeled macrophages was mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Entin-Meer M, Cohen L, Hertzberg-Bigelman E, Levy R, Ben-Shoshan J, Keren G. TRPV2 knockout mice demonstrate an improved cardiac performance following myocardial infarction due to attenuated activity of peri-infarct macrophages. PLoS One. 2017;12(5):e0177132. Figure 4(a)

HEK293T cells transfected with p.G120C-mRFP-ICK were mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Oud MM, Bonnard C, Mans DA, Altunoglu U, Tohari S, Ng AY, et al. A novel ICK mutation causes ciliary disruption and lethal endocrine-cerebro-osteodysplasia syndrome. Cilia. 2016;5:8. Figure 2(a)

Caco-2 cells were exposed to titanium dioxide nanoparticles, stained with AF633 conjugated phalloidin, and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Krüger K, Schrader K, Klempt M. Cellular response to titanium dioxide nanoparticles in intestinal epithelial Caco-2 cells is dependent on endocytosis-associated structures and mediated by EGFR. Nanomaterials. 2017;7:79. Figure 3(b)

Human articular chrondrocytes were stained with anti-collagen II followed by an AF594 conjugated secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Berg V, Sveinbjörnsson B, Bendiksen S, Brox J, Meknas K, Figenschau Y. Human articular chondrocytes express ChemR23 and chemerin; ChemR23 promotes inflammatory signalling upon binding the ligand chemerin21-157. Arthritis Res Ther. 2010;12:R228. Figure 1(a)

Human cancer-associated fibroblasts 120 h post exposure to 18 Gy were stained with anti-53BP1 followed by an AF546 conjugated secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Hellevik T, Pettersen I, Berg V, Winberg JO, Moe BT, Bartnes K, et al. Cancer-associated fibroblasts from human NSCLC survive ablative doses of radiation but their invasive capacity is reduced. Radiat Oncol. 2012;7:59. Figure 1(c)

Human cancer-associated fibroblasts 120 h post exposure to 18 Gy were stained with anti-vinculin followed by an AF488 conjugated secondary antibody and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Hellevik T, Pettersen I, Berg V, Winberg JO, Moe BT, Bartnes K, et al. Cancer-associated fibroblasts from human NSCLC survive ablative doses of radiation but their invasive capacity is reduced. Radiat Oncol. 2012;7:59. Figure 6(a)

Paraffin embedded L. erinacea embryos were stained with CM-DiI and mounted with DAPI Fluoromount-G® (SB Cat. No. 0100-20). Image from Gillis JA, Tidswell OR. The origin of vertebrate gills. Curr Biol. 2017;27:729-32. Figure 3(f)