Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB, IgG2a, Clone: SB146a, Monoclonal

Artikelnummer: SBA-13200-01
Artikelname: Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB, IgG2a, Clone: SB146a, Monoclonal
Artikelnummer: SBA-13200-01
Hersteller Artikelnummer: 13200-01
Alternativnummer: SBA-13200-01
Hersteller: SouthernBiotech
Wirt: Mouse
Kategorie: Antikörper
Applikation: WB, ELISA, IP, FC, ICC, IHC
Konjugation: UNLB (Unconjugated)
Purified Anti-Acetyl-Histone H2A (Lys9) antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, ELISA, western blotting, and immunoprecipitation assays.
Klonalität: Monoclonal
Klon-Bezeichnung: SB146a
Isotyp: IgG2a
Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-BIOT (SB Cat. No. 0103-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H2A (Lys9) (SB Cat No. 13200) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
Trichostatin A (TSA) treated and untreated human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-PE (SB Cat. No. 1030-09).
Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
Trichostatin A (TSA) treated and untreated human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-BIOT (SB Cat. No. 0103-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H2A (Lys9) (SB Cat No. 13200) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
H2AK9ac was immunoprecipitated from total cell lysates from Jurkat cells treated with trichostatin A (TSA) using Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (Cat. No. 13200-01). Total cell lysates from Jurkat cells treated with trichostatin A (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (Cat. No. 13200-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05) secondary antibody and chemiluminescent detection.
Lane 1 - Untreated Lane 2 - 1,000 pM TSA-treated Lane 3 - 200 pM TSA-treated Lane 4 - 40 pM TSA-treated Lane 5 - 8 pM TSA-treated Jurkat cell lysates were treated in a dose dependent manner with trichostatin A (TSA), resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.