Mouse Anti-His-Tag-BIOT, Monoclonal

Produktübersicht

• Artikelname: Mouse Anti-His-Tag-BIOT, Monoclonal
• Artikelnummer: SBA-4603-08
• Hersteller Artikelnummer: 4603-08
• Alternativnummer: SBA-4603-08
• Hersteller: SouthernBiotech
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IP, WB
• Konjugation: Biotin

Produktbeschreibung

Biotin Anti-His-Tag for use in ELISA, western blotting, immunoprecipitation, and immunocytochemistry assays.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [SB194b]
• Isotyp: IgG1
• Formulierung: Purified
• Antibody Type: Secondary Antibody

Bilder

CHO-K1 cells transfected with GFP - N-terminal 6X His-tag were stained with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.

ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).

TEV protease / maltose binding protein (MBP) fusion protein with an internal 6X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) secondary antibody and chemiluminescent detection.

Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.

Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.

CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.