Mouse Anti-His-Tag-BIOT, IgG1, Clone: SB194b, Monoclonal

Artikelnummer: SBA-4603-08
Artikelname: Mouse Anti-His-Tag-BIOT, IgG1, Clone: SB194b, Monoclonal
Artikelnummer: SBA-4603-08
Hersteller Artikelnummer: 4603-08
Alternativnummer: SBA-4603-08
Hersteller: SouthernBiotech
Wirt: Mouse
Kategorie: Antikörper
Applikation: WB, ELISA, ICC, IP
Konjugation: BIOT (Biotin)
Biotin Anti-His-Tag for use in ELISA, western blotting, immunoprecipitation, and immunocytochemistry assays.
Klonalität: Monoclonal
Klon-Bezeichnung: SB194b
Isotyp: IgG1
CHO-K1 cells transfected with GFP - N-terminal 6X His-tag were stained with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
ELISA plate was coated with C-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05).
TEV protease / maltose binding protein (MBP) fusion protein with an internal 6X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) secondary antibody and chemiluminescent detection.
Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08; Clone SB194b) and Clone HIS.H8 conjugated to biotin followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
Tau-410 (2N3R) with an N-terminal 8X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-BIOT (SB Cat. No. 4603-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.