LentiStarter 3.0 Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 2-5 preps

Artikelname:	LentiStarter 3.0 Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 2-5 preps
Artikelnummer:	SBI-LV060A-1
Hersteller Artikelnummer:	LV060A-1
Alternativnummer:	SBI-LV060A-1
Hersteller:	System Biosciences
Kategorie:	Molekularbiologie

Overview LentiStarter 3.0 Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 2-5 preps

Effortlessly introduce lentivirus production into your lab

If you’re new to lentivirus production, SBI’s LentiStarter 3.0 Kit is a great way to get started. With optimized reagents designed to deliver high-titer virus preps, the LentiStarter 3.0 Kit takes the worry out of lentivirus production and is suitable for any application where stable gene delivery and expression is desired.

The LentiStarter 3.0 Kit includes almost everything you need to start producing lentivirus—you supply the HEK293T/TN/FT producer cells and the lentivector and we provide the critical reagents:

pPACK Packaging System (100 µL)
PureFection Transfection Reagent (120 µL)
PEG-it Concentration Reagent (20 mL)
TransDux™ MAX Transduction Reagent (10 reactions*)

But with LentiStarter 3.0, you get more than the kit—you get confidence backed by:

SBI’s well-proven, widely-published lentivirus technology
Optimized reagents that we keep on improving
Deep technical expertise from the scientists that develop our lentivirus production products and perform our lentivirus production services

The Kit includes enough reagents for generating lentivirus from either two 15 cm plates or five 10 cm plates.

*One transduction reaction is defined as 500 µL of total volume in a single well of a 24-well plate

How it works

Kit Contents

pPACK Packaging System

Reliable—generate high titers of transduction-ready pseudoviral particles
Powerful—efficiently deliver and stably express your lentivector constructs in a wide range of mammalian cells
Well-designed—quantify pseudoviral titers and transduction efficiency using a convenient copGFP control
Flexible—choose from FIV and HIV-based lentivector packaging kits

PureFection Transfection Reagent

Flexible—highly effective transfection technology supports a range of applications
Compatible with most cell types
Works with both plasmid DNA and siRNAs
Cost-effective—excellent alternative to expensive, lipid-based products
Safe—nano-based gene delivery with low toxicity
Fast—quick fifteen-minute protocol makes PureFection ideal for high-throughput transfections

PEG-it Concentration Reagent

Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
Cost-effective—contributes to more efficient transductions
Powerful—concentrate lentiviral particles 10- to 100-fold
Scalable—enables isolation of virus from large volumes of media
Safe—supports a range of applications
Found to be non-toxic to all target cell lines tested, including ES cells
Prevent toxic and immunogenic reactions by avoiding accumulation of cellular debris

TransDux™ MAX Transduction Reagent

Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
Non-toxic—no need to change out media after infection
Versatile—works with all types of packaged lentivirus
Flexible—enhances transduction efficiency in a wide variety of cell lines
Broadly compatible—does not interfere with downstream gene expression or functional assays

*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1).

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