LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX)
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Artikelname: | LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) |
Artikelnummer: | SBI-LV340A-1 |
Hersteller Artikelnummer: | LV340A-1 |
Alternativnummer: | SBI-LV340A-1 |
Hersteller: | System Biosciences |
Kategorie: | Molekularbiologie |
LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 10 preps |
Overview LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it, and TransDux MAX)) 10 prepsSimplify large scale lentivirus production with the LentiSuite Basic Kit Get the reagents you need for large scale lentivirus production efficiently bundled into a single kit with the LentiSuite Basic Kit. This kit is ideal for fast-moving labs that don’t need quantitative titer information, and supports generation of high-titer lentivirus particles for any application where stable gene delivery and expression is desired. Compatible with all of SBI’s lentivectors and constructs, as well as all other third-generation lentivectors, the LentiSuite Basic Kit includes optimized reagents to support large-scale virus production—you provide the HEK293T/TN/FT producer cells and lentivector (or purchase them separately) and this kit provides enough critical reagents to support 10 virus preps from 10 cm plates, or 5 virus preps from 15 cm plates: pPACK Packaging System (100 µL)
With the LentiSuite Basic Kit, you get more than the kit—you get confidence backed by: SBI’s well-proven, widely-published lentivirus technology
The Kit includes enough reagents for generating lentivirus from either two 15 cm plates or five 10 cm plates. *One transduction reaction is defined as 500 µL of total volume in a single well of a 24-well plate
How it works
Kit Contents
pPACK Packaging System
Reliable—generate high titers of transduction-ready pseudoviral particles
PureFection Transfection Reagent
Flexible—highly effective transfection technology supports a range of applications
PEG-it Concentration Reagent
Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
TransDux™ MAX Transduction Reagent
Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1). |
Lagerung: | See Manual |