LentiSuite Deluxe Kit (includes HEK293TN Producer Cells, pPACKH1, PureFection, PEG-it, TransDux MAX, & Global UltraRapid Titering Kit)

Artikelname:	LentiSuite Deluxe Kit (includes HEK293TN Producer Cells, pPACKH1, PureFection, PEG-it, TransDux MAX, & Global UltraRapid Titering Kit)
Artikelnummer:	SBI-LV350A-1
Hersteller Artikelnummer:	LV350A-1
Alternativnummer:	SBI-LV350A-1
Hersteller:	System Biosciences
Kategorie:	Zellen/Zellkultur

LentiSuite Deluxe Kit (includes HEK293TN Producer Cells, pPACKH1, PureFection, PEG-it, TransDux MAX, & Global UltraRapid Titering Kit) 10 preps

Streamline large scale lentivirus production with our comprehensive LentiSuite Deluxe Kit

For production and other labs that produce lentivirus particles at large scales and need to obtain quantitative titer information, SBI has bundled everything you need into the LentiSuite Deluxe Kit. Get everything you need to generate high-titer lentivirus particles for any application where stable gene delivery and expression is desired.
Compatible with all of SBI’s lentivectors and constructs, as well as all other third-generation lentivectors, the LentiSuite Deluxe Kit includes optimized reagents to support large-scale virus production, including HEK293T/TN/FT producer cells and the Global UltraRapid™ Titering Kit. This kit provides enough critical reagents to support 10 virus preps from 10 cm plates, or 5 virus preps from 15 cm plates:

HEK293TN Producer Cell Line (1 vial)
pPACK Packaging System (200 µL)
PureFection Transfection Reagent (1 ml)
PEG-it Concentration Reagent (100 mL)
TransDux™ MAX Transduction Reagent (100 reactions*)
Global UltraRapid Titering Kit (1 kit)

With the LentiSuite Deluxe Kit, you get more than the kit—you get confidence backed by:

    SBI’s well-proven, widely-published lentivirus technology
    Optimized reagents that we keep on improving
    Deep technical expertise from the scientists that develop our lentivirus production products and perform our lentivirus production services

*One transduction reaction is defined as 500 µL of total volume in a single well of a 24-well plate.

How It Works

Everything you need for large-scale lentivirus production

The LentiSuite Deluxe Kit includes optimized reagents for large-scale lentivirus production when quantitative titer information is not needed

Kit Contents

HEK293TN Producer Cell Line

pPACK Packaging System

Reliable—generate high titers of transduction-ready pseudoviral particles
Powerful—efficiently deliver and stably express your lentivector constructs in a wide range of mammalian cells
Well-designed—quantify pseudoviral titers and transduction efficiency using a convenient copGFP control
Flexible—choose from FIV and HIV-based lentivector packaging kits

PureFection Transfection Reagent

Flexible—highly effective transfection technology supports a range of applications
Compatible with most cell types
Works with both plasmid DNA and siRNAs
Cost-effective—excellent alternative to expensive, lipid-based products
Safe—nano-based gene delivery with low toxicity
Fast—quick fifteen-minute protocol makes PureFection ideal for high-throughput transfections

PEG-it Concentration Reagent

Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
Cost-effective—contributes to more efficient transductions
Powerful—concentrate lentiviral particles 10- to 100-fold
Scalable—enables isolation of virus from large volumes of media
Safe—supports a range of applications
Found to be non-toxic to all target cell lines tested, including ES cells
Prevent toxic and immunogenic reactions by avoiding accumulation of cellular debris

TransDux™ MAX Transduction Reagent

Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
Non-toxic—no need to change out media after infection
Versatile—works with all types of packaged lentivirus
Flexible—enhances transduction efficiency in a wide variety of cell lines
Broadly compatible—does not interfere with downstream gene expression or functional assays

Global UltraRapid Titering Kit

Rapid—get data more quickly than what’s possible with conventional p24 ELISA titer kits (< 3 hours from cells-to-titer!)
Accurate—relies on highly quantitative qPCR measurement of lentiviral titers
Easy—isolation and/or concentration of genomic DNA is NOT required
Quantitative—built-in reference control simplifies normalization.
Dynamic—obtain titering quantitation up to 15 MOI
Compatible—works with most WPRE-containing lenti-constructs (g. pLVCT-H1, pLVTHM, pGIPz, HiPerform™ and all SBI lentivectors)

*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1).

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