Rainbow Calibration Particles, EUROFLOW, 8 peaks, 1E7/mL, 3.0-3.4µm

Produktübersicht

• Artikelname: Rainbow Calibration Particles, EUROFLOW, 8 peaks, 1E7/mL, 3.0-3.4µm
• Artikelnummer: SPH-RCP-30-5A(EUROFLOW)
• Hersteller Artikelnummer: RCP-30-5A (EUROFLOW)
• Alternativnummer: SPH-RCP-30-5A(EUROFLOW)
• Hersteller: Spherotech
• Kategorie: Sonstiges

Zusatzinformationen

SPHERO™ Calibration Particles are designed for routine calibration of flow cytometers. They are used extensively by many laboratories for QC and long term performance tracking. In addition, they are used for routine alignment and calibration in fluorescence and confocal fluorescence microscopy.

 

SPHERO™ Rainbow Calibration Particles

• Contains multiple fluorophores incorporated in the same particle to be used in multiple channels of the flow cytometer
• Available with different fluorescent intensities on the same size particles
• Stable for several years when stored properly
• Withstand freeze-thaw cycles; diluted particles can be stored frozen for later use
• Can be sanitized by treating with 70% ethanol or other antibiotic agents

The RCPs provide a reliable and reproducible particle mixture for calibrating flow cytometers. They are very stable since the fluorochromes are entrapped within the particles instead of being located on the surface. In addition, Spherotech uses fluorophores that are non-spectral matching to the commonly used fluorophores such as FITC, PE or PE-Cy5. As a result, the RCPs are stable in terms of fluorescence.

The RCPs are convenient and affordable to use for long term performance tracking or routine calibration. They are packaged in a dropper bottle to facilitate dispensing and storage. Dilution of a few drops of the particles from the dropper bottle to 1 mL of a diluent will provide adequate particle concentration for flow cytometer calibration.

 

Downloadable NOTE Rainbow Calibration Particles & SDS - Rainbow Particles

 

SPHERO™ Rainbow Calibration Particles for EuroFlow™ Standardization Guidelines

Rainbow Calibration Particles, 8 peaks, Cat. No. RCP-30-5A(EuroFlow), are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers. RCP-30-5A(EuroFlow) provide target mean fluorescence intensities (MFI) to aid in the full standardization in 8-color flow cytometric immunophenotyping panel of normal and malignant leukocytes in bone marrow and blood.

 

Available Rainbow Calibration Particles (RCP):

Catalog No.	Description	Concentration	Nominal Size	Package Size
SPH-RCP-20-5	RCP, 4 peaks	107/mL	1.8-2.2 µm	5 mL
SPH-RCP-30-5	RCP, 6 peaks	107/mL	3.0-3.4 µm	5 mL
SPH-RCP-30-5A	RCP, 8 peaks	107/mL	3.0-3.4 µm	5 mL
SPH-RCP-30-20A	RCP, 8 peaks	107/mL	3.0-3.4 µm	20 mL
SPH-RCP-30-5A-2	RCP, 2nd peak from the 8 peak set	107/mL	3.0-3.4 µm	5 mL
SPH-RCP-30-5A(EUROFLOW)	RCP, 8 peaks, for EUROFLOW	107/mL	3.0-3.4 µm	5 mL
SPH-RCP-32-5	RCP, 6 peaks	107/mL	3.2 (+/- 0.1) µm	5 mL
SPH-RCP-35-5	RCP, 4 peaks	107/mL	3.5-4.0 µm	5 mL
SPH-RCP-60-5	RCP, 6 peaks	107/mL	6.0-6.4 µm	5 mL
SPH-RCP-60-20	RCP, 6 peaks	107/mL	6.0-6.4 µm	20 mL