The Cleavage and Tag Control Protein is a versatile tool consisting of various elements and cleavage sites for different proteases. Furthermore, it's equipped with multiple tags to serve as positive control for Western Blot analyses or functional assays.
Specifications:
- Product Name: Cleavage and Tag Control Protein
- Catalog No.: P2020-141
- Synonyms: control protein, reference protein, cleavage control protein, multiple tag control protein, universal reference protein
- Species: artificial
- Tags: Twin-Strep, HA, T7, Avi, FLAG, and His-tag; N-terminal MBP and C-terminal sfGFP fusion
- Expression Host: E. coli
- Formulation: PBS; pH 7.4
- Format: lyophilized, stored at -80 °C and shipped at ambient temperature. We recommend reconstituting the protein in ddH2O. In case of 10 µg, add 11 µl ddH2O.
- Purity: > 99 % (measured by densitometry of Coomassie stained gel, see below)
The Cleavage and Tag Control Protein is a versatile protein consisting of various cleavage sites for proteases including TEV, Enterokinase, Thrombin, Factor Xa and PreCission. Protease cleavage results in generation of two or three fragments that have significantly reduced molecular weights, which are as follows:
- TEV cleavage: 42.8 kDa and 47.7 kDa
- Enterokinase: 43.5 kDa, 20.1 kDa and 26.9 kDa
- Thrombin: 43.9 kDa and 46.6 kDa
- Factor Xa: 44.3 kDa and 46.2 kDa
- PreCission: 45.3 kDa and 45.3 kDa
Moreover, the 90.5 kDa fusion protein is equipped with multiple tags such as Twin-Strep-, HA-, FLAG-, and His-tag, for instance, that may serve as positive control performing specific Western Blot analyses or any functional assay. Since the protein is fused to GFP, it is additionally applicable as fluorescence control. Thus, the Cleavage and Tag Control Protein provides an excellent tool to monitor protease cleavage reactions and specific Western Blot analyses as well as fluorescence experiments.
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SDS-PAGE/Coll. Coomassie
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Histogram of marked lane in gel picture
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Test Cleavage
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Description of Test Cleavage
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Test Cleavage
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Description for Test Cleavage
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