E2 Scan Kit version 2

Artikelnummer: UBI-67-0005-001
Artikelname: E2 Scan Kit version 2
Artikelnummer: UBI-67-0005-001
Hersteller Artikelnummer: 67-0005-001
Alternativnummer: UBI-67-0005-001
Hersteller: Ubiquigent
Kategorie: Kits/Assays

The E2scan™ Kit version 2 is designed to facilitate the identification of E2 conjugating enzymes which support the ubiquitylation of substrate proteins catalysed by an E3 ligase and/or the auto-ubiquitylation of the E3 ligase itself.

The kit contains an E2scan™ plate: A panel of 34 E2 conjugating enzymes arrayed in duplicate across a 96-well plate plus all the components (E1 enzyme, control E3 ligase, ubiquitin, ATP, assay buffer) required to perform a complete substrate ubiquitylation or auto-ubiquitylation assay using your E3 ligase and/or E3 ligase/substrate combination of choice. Through the addition of a substrate mix (E1, E3, substrate, and ubiquitin) and then ATP to the wells of an E2scan™ plate you can screen the E2 conjugating enzyme panel in just one hour. Reaction products may be analysed as required, for example by SDS-PAGE or Western blotting using specific antibodies.

Utility of the E2scan™ Kit version 2

Ubiquigent’s E2scan™ Kit version 2 will help you to address many fundamental questions in your ubiquitin system research. Potential kit applications are listed below:

  • Identify the E2(s) which couple with your E3 ligase of interest.
  • Determine which E2s support auto and/or substrate-ubiquitylation with your E3.
  • Help define mechanisms of E3/E2 interactions through the comparison of E2scan™ data between wild-type and modified versions of the E3 under evaluation.
  • Investigate E3 intra-family E2 selectivity.
  • Explore the E2 ‘priming theory’.
  • Explore potential E2 inhibitors and/or activators.
NCBI: <a href=https://www.ncbi.nlm.nih.gov/gene/N/A target=_blank rel=nofollow>N/A