DUB Scan Kit

Produktübersicht

• Artikelname: DUB Scan Kit
• Artikelnummer: UBI-67-0006-001
• Hersteller Artikelnummer: 67-0006-001
• Hersteller: Ubiquigent
• Kategorie: Proteine/Peptide

Zusatzinformationen

Background:

The DUBscan™ kit has been flexibly designed for many potential applications. One application that might be of greatest interest is the identification of DUB enzymes which support the deubiquitylation of substrate proteins.

The kit contains a DUBscan™ plate (a panel of DUBs arrayed across 47 wells of a 96 well plate) plus a control ubiquitylation reaction mix for performing both positive and negative control deubiquitylation reactions. Through the addition of your own DUB substrate of interest to the wells of a DUBscan™ plate you can screen the DUB enzyme panel in just one hour. Reaction products may be analysed as required, for example by SDS-PAGE and Western blotting using specific antibodies. One kit contains all the reagents and buffers necessary to perform a DUBscan™ of the panel of 47 DUB assay wells.

Utility of the DUBscan™ Kit

• Examine the cleavage of in vitro ubiquitylated substrates generated using different E2/E3 combinations (refer to the Ubiquigent E2scan™ kit for identifying E2s that ‘couple’ with your E3 of interest)
• Examine the cleavage of in vivo ubiquitylated substrates after capture from a cell lysate (refer to Ubiquigent ubiquitin chain binding proteins for capturing ubiquitylated proteins
• Investigate DUB ubiquitin-ubiquitin linkage cleavage specificity (refer to Ubiquigent di-ubiquitin and ubiquitin chains for use as substrates
• Examine the relative position of cleavage within ubiquitin chains (distal versus proximal)
• Explore mono and poly-deubiquitylation DUB specificity (refer to specific Ubiquigent E2s – such as Ube2W – for mono-ubiquitylating E2s
• Explore potential DUB inhibitors and/or activators
• Identify novel DUB binding proteins
• Explore how DUBs may interact with and modify the activity of other ubiquitin system proteins such as E2s and vice versa