Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions. Recombinant 6xHis-TEV was expressed in E. Coli and purified to near homogeneity. A Ser219Asn (S219N) substitution was introduced that has no effect on TEV protease activity, but inhibits auto-cleavage and thus, stabilizing TEV protease during incubation and long-term storage.
Molekulargewicht:
27 kDa
Puffer:
100 mM Tris, pH 8.5 at 4 0C, 500 mM NaCl, 5mM DTT, 0.5 mM EDTA and 50% Glycerol
Quelle:
E. coli recombinant
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