UCHL1, Active, Recombinant, Human, His-Tag (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1)

Produktübersicht

• Artikelname: UCHL1, Active, Recombinant, Human, His-Tag (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1)
• Artikelnummer: USB-137391
• Hersteller Artikelnummer: 137391
• Alternativnummer: USB-137391-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-terminal glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and thier tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5. Source: Recombinant corresponding to aa2-224 from human UCHL1, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~23.4kD Applications: Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Molekulargewicht: 23.4
• NCBI: 004181
• UniProt: P09936
• Reinheit: Highly Purified (~95%)
• Formulierung: Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 225mM imidazole, 10% glycerol, 3mM DTT.