Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma. Intended Use: For quantitative detection of human CCL18 in cell culture supernates and serum. Range: 7.8-500pg/ml Sensitivity: < 10pg/ml Specificity: Natural and recombinant human CCL18 Crossreactivity: No detectable crossreactivity with other relevant proteins Kit Components: 143747A: Microtiter Strip, 1x96 wells 143747B: CCL18 Standard, 2x10ng 143747C: Anti-human CCL18 (Biotin), 130ul USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution) 143697: Sample diluent buffer, 1x30ml 143698: Antibody diluent buffer, 1x12ml 143699: ABC diluent buffer, 1x12ml 143700: TMB color developing agent, 1x10ml 143701: TMB stop solution, 2N H2SO4, 1x10ml Storage and Stability: Store *143747B powder at 4C. Once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add 100ul of samples and standards and incubate the plate at 37C for 90 min. Do not wash. 2. Add 100ul biotinylated antibody and incubate the plate at 37C for 60 min. Wash plate 3 times with 0.01M TBS or PBS. 3. Add ABC working solution and incubate the plate at 37C for 30min. Wash plate 5 times with 0.01M TBS or PBS. 4. Add TMB color developing agent and incubate the plate at 37C in dark for 20-25 min. 5. Add 100ul TMB stop solution and read.
