Fibroblast Growth Factor 21 (FGF21) BioAssay(TM) ELISA Kit, Human
Artikelnummer:
USB-143831
Hersteller Artikelnummer:
143831
Alternativnummer:
USB-143831-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Uniprot ID: Q9NSA1 Entrez Gene ID: 26291 Intended Use: Fibroblast Growth Factor 21 (FGF21) BioAssay(TM) ELISA Kit, Human is a sandwich ELISA for quantitative detection of human FGF21 in cell culture supernates, serum and plasma (heparin, EDTA). Range: 31.2-2000pg/ml Sensitivity: <10pg/ml Specificity: Natural and recombinant human FGF21 Crossreactivity: No detectable crossreactivity with other relevant proteins Kit Components: 143831A: Microtiter Strips, 1x96 wells *143831B: FGF21 Standard, 2x1 vial 143831C: Anti-human FGF21 (Biotin), 1x130ul USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution) 143697: Sample Diluent Buffer, 1x30ml 143698: Antibody Diluent Buffer, 1x12ml 143699: ABC Diluent Buffer, 1x12ml 143700: TMB Color Developing Agent, 1x10ml 143701: TMB Stop Solution, 2N H2SO4, 1x10ml Assay Summary: 1. Add samples and standards and incubate the plate at 37C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37C for 60 minutes. Wash plate 3 times with 0.01M TBS. 3. Add ABC working solution and incubate the plate at 37C for 30 minutes. Wash plate 5 times with 0.01M TBS. 4. Add TMB color developing agent and incubate the plate at 37C in dark for 25-30 minutes. 5. Add TMB stop solution and read. Storage and Stability: Store *143831B powder at 4C. Once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
