Sandwich ELISA quantitative detection kit for Human Growth Hormone in cell culture supernates, serum and plasma (heparin, EDTA). Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22kD. Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 2 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: B1A4G6 Entrez Gene ID: 2688 Kit Components: 96-well plate pre-coated with anti-Growth Hormone, Human Lyophilized recombinant Growth Hormone, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
