Interleukin 5, Mouse, BioAssay(TM) ELISA Kit (IL-5, Interleukin-5, B Cell Differentiation Factor I, T-cell Replacing Factor, TRF)
Artikelnummer:
USB-143928
Hersteller Artikelnummer:
143928
Alternativnummer:
USB-143928-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Sandwich ELISA quantitative detection kit for Mouse IL-5 in cell culture supernates and serum. Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation.1 The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators.2 IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3 Human and mouse of IL-5 share 70% amino acid sequence homology. Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 2 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P04401 Entrez Gene ID: 16191 Kit Components: 96-well plate pre-coated with anti-IL-5, Mouse Lyophilized recombinant IL-5, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
