PIN1 (Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Prolyl isomerase, Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1, Protein NIMA interacting 1, Rotamase Pin1, UBL 5), Rabbit
Artikelnummer:
USB-168437
Hersteller Artikelnummer:
168437
Alternativnummer:
USB-168437-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IHC, IP, WB
Immunogen:
Synthetic peptide corresponding to an internal sequence of human Pin1. Species sequence homology: mouse and rat
Pin1 (peptidylprolyl cis/trans isomerase NIMA-interacting 1 protein) is an essential peptidylprolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond and also catalyzes pSer/Thr-Pro cis/trans isomerizations. Pin1 shows a nuclear localization. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:2500-1:10,000 Western Blot: 1:500-1:3000 Immunoprecipitation: 1:100 Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
