RLBP1 (Retinaldehyde Binding Protein 1, CRALBP, Cellular retinaldehyde-binding protein), Rabbit
Artikelnummer:
USB-364917
Hersteller Artikelnummer:
364917
Alternativnummer:
USB-364917-200
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Met1-Phe317 of human RLBP!, expressed in E. coli.
Retinaldehyde-binding protein 1 is a 36kD water-soluble protein which carries 11-cis- retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens. CRALBP is not expressed in photoreceptors but is abundant in the retinal pigment epithelium (RPE) and Muller cells of the neuroretina, where it carries 11-cis-retinol and 11-cis-retinaldehyde. When expressed in bacteria, recombinant CRALBP containing the R150Q substitution was less soluble than wildtype recombinant CRALBP. Mutant CRALBP was purified and found to lack the ability to bind 11-cis-retinaldehyde. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
