FZD4, also known as frizzled-4, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. This pathways seem to involve interactions with G-proteins. It is involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Recombinant protein corresponding to aa37-222 from human FZD4, fused to hIgG-His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~48.3kD (428aa), 50-70kD (SDS-PAGE under reducing conditions) Amino Acid Sequence: ADPFGDEEER RCDPIRISMC QNLGYNVTKM PNLVGHELQT DAELQLTTFT PLIQYGCSSQ LQFFLCSVYV PMCTEKINIP IGPCGGMCLS VKRRCEPVLK EFGFAWPESL NCSKFPPQND HNHMCMEGPG DEEVPLPHKT PIQPGEECHS VGTNSDQYIW VKRSLNCVLK CGYDAGLYSR SAKEFTDIWL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
