Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes.It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-210 of mouse Ak1 protein, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~25.5kD (233aa), Confirmed by MALDI-TOF Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK Specific Activity: >150units/mg. One unit will convert 2umoles of ADP to ATP + AMP/minute at pH 7.5 at 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
