CHGA, also known as chromogranin-A isoform 1 preproprotein, is a member of neuroendocrine secretory proteins of the chromogranin / secretogranin family. It is a precursor of several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. This peptide negatively regulates the neuroendocrine function of autocrine or nearby cells (paracrine). This protein induces and promotes the production of secretory granules containing insulin in pancreatic islet beta cells. Recombinant human CHGA, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa19-457 of human CHGA, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~50kD (448aa), ~50-70kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADLLPVNSPM NKGDTEVMKC IVEVISDTLS KPSPMPVSQE CFETLRGDER ILSILRHQNL LKELQDLALQ GAKERAHQQK KHSGFEDELS EVLENQSSQA ELKEAVEEPS SKDVMEKRED SKEAEKSGEA TDGARPQALP EPMQESKAEG NNQAPGEEEE EEEEATNTHP PASLPSQKYP GPQAEGDSEG LSQGLVDREK GLSAEPGWQA KREEEEEEEE EAEAGEEAVP EEEGPTVVLN PHPSLGYKEI RKGESRSEAL AVDGAGKPGA EEAQDPEGKG EQEHSQQKEE EEEMAVVPQG LFRGGKSGEL EQEEERLSKE WEDSKRWSKM DQLAKELTAE KRLEGQEEEE DNRDSSMKLS FRARAYGFRG PGPQLRRGWR PSSREDSLEA GLPLQVRGYP EEKKEEEGSA NRRPEDQELE SLSAIEAELE KVAHQLQALR RGHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
