FCGR3B, also known as low affinity immunoglobulin gamma Fc region receptor III-B isoform 2, is a member of Ig super family. It encodes a glycosylphosphatidylinositol (GPI)-anchored protein that is expressed constitutively by neutrophils and after gamma-interferon stimulation by eosinophils. This protein binds to complex or aggregated IgG and monomeric IgG. Unlike III-A, it cannot mediate antibody-dependent cytotoxicity and phagocytosis. It can be a trap of immune complexes in peripheral blood circulation that does not activate neutrophils. Recombinant human FCGR3B, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa21-200 of human FCGR3B, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~21.4kD (188aa), ~28-40kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: EDLPKAVVFL EPQWYSVLEK DSVTLKCQGA YSPEDNSTQW FHNENLISSQ ASSYFIDAAT VNDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKDRKY FHHNSDFHIP KATLKDSGSY FCRGLVGSKN VSSETVNITI TQGLAVSTIS VEHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
