Prdx2, also known as peroxiredoxin-2, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Prdx2 may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. If Prdx2 protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimers or DNA damage leading to cancer. Recombinant mouse Prdx2, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-198 of mouse Prdx2, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~24.3kD (222aa), Confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASGNA QIGKSAPDFT ATAVVDGAFK EIKLSDYRGK YVVLFFYPLD FTFVCPTEII AFSDHAEDFR KLGCEVLGVS VDSQFTHLAW INTPRKEGGL GPLNIPLLAD VTKSLSQNYG VLKNDEGIAY RGLFIIDAKG VLRQITVNDL PVGRSVDEAL RLVQAFQYTD EHGEVCPAGW KPGSDTIKPN VDDSKEYFSK HN Specific Activity: >700pmol/min/ug, activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C/minute. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
