SEMA7A, also known as semaphorin-7A isoform 1, is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. It promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. It plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. It promotes production of proinflammatory cytokines by monocytes and macrophages. Recombinant human SEMA7A, fused to hIgG-His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa45-648 of human SEMA7A, fused to hIgG-His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~95.7kD (846aa), ~70-100kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPQGHLRSG PRIFAVWKGH VGQDRVDFGQ TEPHTVLFHE PGSSSVWVGG RGKVYLFDFP EGKNASVRTV NIGSTKGSCL DKRDCENYIT LLERRSEGLL ACGTNARHPS CWNLVNGTVV PLGEMRGYAP FSPDENSLVL FEGDEVYSTI RKQEYNGKIP RFRRIRGESE LYTSDTVMQN PQFIKATIVH QDQAYDDKIY YFFREDNPDK NPEAPLNVSR VAQLCRGDQG GESSLSVSKW NTFLKAMLVC SDAATNKNFN RLQDVFLLPD PSGQWRDTRV YGVFSNPWNY SAVCVYSLGD IDKVFRTSSL KGYHSSLPNP RPGKCLPDQQ PIPTETFQVA DRHPEVAQRV EPMGPLKTPL FHSKYHYQKV AVHRMQASHG ETFHVLYLTT DRGTIHKVVE PGEQEHSFAF NIMEIQPFRR AAAIQTMSLD AERRKLYVSS QWEVSQVPLD LCEVYGGGCH GCLMSRDPYC GWDQGRCISI YSSERSVLQS INPAEPHKEC PNPKPDKAPL QKVSLAPNSR YYLSCPMESR HATYSWRHKE NVEQSCEPGH QSPNCILFIE NLTAQQYGHY FCEAQEGSYF REAQHWQLLP EDGIMAEHLL GHACALALEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
