SFTPD, also known as pulmonary surfactant-associated protein D, is a member of the collectin family of innate immune modulators. It contributes to the lungs defense against inhaled microorganisms, organic antigens and toxins. This protein interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. Recombinant human SFTPD, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa22-375 of human SFTPD, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~36.5kD (363aa), ~40-57kD (SDS-PAGE under reducing conditions.) Amino Acid Sequence: ADPEMKTYSH RTMPSACTLV MCSSVESGLP GRDGRDGREG PRGEKGDPGL PGAAGQAGMP GQAGPVGPKG DNGSVGEPGP KGDTGPSGPP GPPGVPGPAG REGPLGKQGN IGPQGKPGPK GEAGPKGEVG APGMQGSAGA RGLAGPKGER GVPGERGVPG NTGAAGSAGA MGPQGSPGAR GPPGLKGDKG IPGDKGAKGE SGLPDVASLR QQVEALQGQV QHLQAAFSQY KKVELFPNGQ SVGEKIFKTA GFVKPFTEAQ LLCTQAGGQL ASPRSAAENA ALQQLVVAKN EAAFLSMTDS KTEGKFTYPT GESLVYSNWA PGEPNDDGGS EDCVEIFTNG KWNDRACGEK RLVVCEFHHH HHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
