TNFRSF10B, also known as tumor necrosis factor receptor superfamily member 10B isoform 1, is a member of receptors for the cytotoxic ligand TNFSF10/TRAIL. It can be activated by tumor necrosis factor-related apoptosis inducing ligand, and transduces an apoptosis signal. This protein promotes the activation of NF-kappa-B. Defects in this protein causes of head and neck squamous cell carcinomas. Recombinant human TNFRSF10B, fused to hIgG-His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa56-210 of human TNFRSF10B, fused to hIgG-His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~43.9kD (394aa), ~40-57kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: ITQQDLAPQQ RAAPQQKRSS PSEGLCPPGH HISEDGRDCI SCKYGQDYST HWNDLLFCLR CTRCDSGEVE LSPCTTTRNT VCQCEEGTFR EEDSPEMCRK CRTGCPRGMV KVGDCTPWSD IECVHKESGT KHSGEVPAVE ETVTSSPGTP ASPCSLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH Biological Activity: Measured by its ability to inhibit cytotoxicity assay using Jurkat human T lymphocyte. The ED50 for this effect less or equal to 5ng/ml with TRAIL. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
