Dulbeccos Phosphate Buffered Saline (PBS) (10X) w/CaCl2, MgCl2 (Powder)

Produktübersicht

• Artikelname: Dulbeccos Phosphate Buffered Saline (PBS) (10X) w/CaCl2, MgCl2 (Powder)
• Artikelnummer: USB-D9821
• Hersteller Artikelnummer: D9821
• Alternativnummer: USB-D9821-10,USB-D9821-25,USB-D9821-50,USB-D9821-100
• Hersteller: US Biological
• Kategorie: Biochemikalien

Produktbeschreibung

Dulbeccos Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process. Also Available: D9819: Dulbeccos Phosphate Buffered Saline (PBS) (Powder) D9820: Dulbeccos Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder) D9820-04: Dulbeccos Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder) Dulbeccos Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder) D9821: Dulbeccos Phosphate Buffered Saline (PBS) (10X) (Powder) D9822: Dulbeccos Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder) D9823: Dulbeccos Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder) Appearance: White, homogeneous, free flowing powder Solubility (1X): Colorless, clear, complete. Some precipitate may be observed as a 10X due to excess concentration of salts. Sterile filter after solubilization. pH: As Reported Endotoxin: 1EU/ml Formulation: Components g/L Calcium Chloride2H2O 1.325 Magnesium Chloride6H2O 1.00 Potassium Chloride 2.00 Potassium Phosphate Mono 2.00 Sodium Chloride 80.00 Sodium Phosphate Dibasic 11.50 Total: 97.8g/L Directions: To make a 10X solution: Dissolve 97.8g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers.. Note: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization. USB recommends the preparation of this product as a 1X solution. To make a 1X solution: Dissolve 9.78g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.