Purified MEK from mouse T cells and recombinant MEK1.
MEK (MAP kinase or ERK kinase) is also known as MAP kinase kinase (MAPKK). MEK1 is a dual tyrosine/threonine kinase that phosphorylates and activates MAP kinase (ERK1 and ERK2). MEK1 has a molecular weight of 43.5kD and contains 393 amino acids. MEK1 is phosphorylated and activated by serine/threonine kinases via 2 different pathways(3). These kinases include, c-raf-1, v-raf-1, c-Mos, and MEKK. MEKK shares homology with yeast kinases Ste11 and byr2. MEK2 has been identified and cloned(4). MEK2 differs from MEK1 in the amino terminal region and between the conserved kinase subdomains 9 and 10. The MEK1 murine gene is found on chromosome 9 the MEK2 gene on chromosome 10. It is likely that more members of the MEK family exist. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Immunoprecipitation: 2-5ug Optimal dilutions to be determined by the researcher. Positive Control: Mouse brain cell lysates Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3G188]
Isotyp:
IgG1
Reinheit:
Purified
Formulierung:
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
