Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound, 2 ml

Produktübersicht

• Artikelname: Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound, 2 ml
• Artikelnummer: VEC-AL-1143
• Hersteller Artikelnummer: AL-1143
• Alternativnummer: VEC-AL-1143
• Hersteller: Vector Laboratories
• Kategorie: Sonstiges

Produktbeschreibung

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound, 2 ml

Zusatzinformationen

Description

Agarose bound Erythrina cristagalli lectin is prepared using 4% agarose beads. Erythrina cristagalli lectin consists of two different subunits of approximately 28 kDa and 26 kDa. The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin.

Features:

• Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons
• Bead diameter ranges in size from 45-165 microns
• Matrix is stable in solutions at pH 3-11 as well as many organic solvents
• Immobilized lectins are prepared using affinity purified lectins
• Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
• Hydrophilic spacer arm is inserted between the lectin and the matrix
• Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
• No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
• Product supplied as a 1:1 suspension in buffer
• Inhibiting/Eluting Sugar: 200 mM lactose or Glycoprotein Eluting Solution (ES-1100)

 

Specifications

• Unit Size: 2 ml
• Applications: Glycobiology, Affinity Chromatography
• Recommended Storage: 2-8 °C DO NOT FREEZE
• Solution: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM lactose, 0.08% sodium azide
• Recommended Usage: Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.2 M lactose can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
• Matrix Conjugate: Lectins
• Sugar Specificity: Galactose, N-Acetylgalactosamine, Lactose
• Conjugate: Agarose

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