Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml

Produktübersicht

• Artikelname: Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml
• Artikelnummer: VEC-AL-1233
• Hersteller Artikelnummer: AL-1233
• Alternativnummer: VEC-AL-1233
• Hersteller: Vector Laboratories
• Kategorie: Sonstiges

Produktbeschreibung

Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml

Zusatzinformationen

Description

Agarose bound Vicia villosa lectin is prepared using our affinity-purified lectins. This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). 

Features:

• Bead diameter ranges in size from 45-165 microns
• Matrix is stable in solutions at pH 3-11 as well as many organic solvents
• Immobilized lectins are prepared using affinity purified lectins
• Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
• No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
• Product supplied as a 1:1 suspension in buffer
• Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine or Glycoprotein Eluting Solution (ES-2100)

 

Specifications

• Unit Size: 2 ml
• Applications: Glycobiology, Affinity Chromatography
• Recommended Storage: 2-8 °C DO NOT FREEZE
• Solution: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose, 0.08% sodium azide
• Recommended Usage: Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-2100. Alternatively, 200 mM N-acetylgalactosamine can be used.Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
• Matrix Conjugate: Lectins
• Sugar Specificity: N-Acetylgalactosamine
• Conjugate: Agarose