Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding.
Biotinylated peanut agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
DO NOT VORTEX. For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. Use of buffers containing 0.1 mMCaCl2 and 0.01 mM MnCl2 is recommended. Inhibiting/Eluting Sugar: 200 mM galactose (S-9003).
Recommended Storage
2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar
Inhibiting/Eluting Sugar: 200 mM galactose (S-9003).
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2
Concentration
5 mg active conjugate/ml
Conjugate
Biotinylated
Sugar Specificity
Galactose
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