Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated, 2 mg

Artikelnummer: VEC-B-1215
Artikelname: Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated, 2 mg
Artikelnummer: VEC-B-1215
Hersteller Artikelnummer: B-1215
Alternativnummer: VEC-B-1215
Hersteller: Vector Laboratories
Kategorie: Sonstiges
Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated, 2 mg

Description

Griffonia (Bandeiraea) Simplicifolia Lectin II is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. 

Biotinylated GSL II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

 

Specifications

 
Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage Reconstitute biotinylated lectin by addition 1 ml of water.The resulting solution will have the following composition:10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 10 mM N-Acetylglucosamine.For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Conjugate Biotinylated
Sugar Specificity N-Acetylglucosamine