Biotinylated Anti-Avidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding avidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Our antibodies to avidin are produced in goats using our highly purified avidin and isolated by affinity chromatography. Anti-Avidin does not bind streptavidin and Anti-Streptavidin does not recognize avidin. These antibodies provide opportunities to significantly amplify signals in many applications.
Specifications
Unit Size
0.5 mg
Applications
Immunofluorescence, In situ hybridization
Recommended Storage
2-8 °C; Store frozen for long term storage
Recommended Usage
Reconstitute by adding 1 ml water.The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 1-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection Target
Avidin
Conjugate
Biotinylated
Host Species
Goat
Format
Lyophilized
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
