Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins, 100 ml

Produktübersicht

• Artikelname: Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins, 100 ml
• Artikelnummer: VEC-ES-2100
• Hersteller Artikelnummer: ES-2100
• Alternativnummer: VEC-ES-2100
• Hersteller: Vector Laboratories
• Kategorie: Sonstiges

Produktbeschreibung

Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins, 100 ml

Zusatzinformationen

Description

Features:

• Convenient, ready-to-use solutions
• Superior recovery over standard sugar solutions
• Maximize the yield of eluted glycoproteins
• Preserve the activity of the agarose-bound lectins for repeated use
• Elution in neutral pH range
• All components can be subsequently removed by dialysis

 

Specifications

• Unit Size: 100 ml
• Applications: Glycobiology, Affinity Chromatography
• Recommended Usage: 1)Wash lectin agarose column to remove unbound proteins with HEPES- or TRIS-Buffered saline (HBS, TBS), pH 7.5, until OD280nm reaches background level. 2)Apply Glycoprotein Eluting Solution and allow to flow through the column by gravity.Note: Do not apply pressure to speed elution because this may reduce the percent recovery of the bound glycoprotein.Complete elution may take up to 10 column volumes.* 3) Following elution, the agarose lectin column is ready for reuse after equilibrating with 2-4 column volumes of HBS or TBS, pH 7.5. *These results may not be typical for other glycoproteins, since oligosaccharide structures of glycoproteins vary significantly.The eluting solution has a high osmolarity.If downstream applications are adversely affected by salt, eluted glycoproteins may require dialysis or gel filtration.
• Recommended Storage: Room Temperature

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