Wheat Germ Agglutinin (WGA), Fluorescein, 10 mg

Artikelnummer: VEC-FL-1021-10
Artikelname: Wheat Germ Agglutinin (WGA), Fluorescein, 10 mg
Artikelnummer: VEC-FL-1021-10
Hersteller Artikelnummer: FL-1021-10
Alternativnummer: VEC-FL-1021-10
Hersteller: Vector Laboratories
Kategorie: Sonstiges
Wheat Germ Agglutinin (WGA), Fluorescein, 10 mg

Description

The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).

Fluorescein labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

 

Specifications

 
Unit Size 10 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylglucosamine