Dolichos biflorus agglutinin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
Fluorescein labeled Dolichos biflorus agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Specifications
Unit Size
2 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage
2-8 °C
Maximum Excitation
495-500 nm
Maximum Emission
514-521 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration
FL-1031-2 (2 mg active conjugate/ml)
FL-1031-5 (5 mg active conjugate/ml)
Conjugate
Fluorescein
Color of Fluorescence
Green
Sugar Specificity
N-Acetylgalactosamine
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
