Lens Culinaris Agglutinin (LCA), Fluorescein, 5 mg

Artikelnummer: VEC-FL-1041
Artikelname: Lens Culinaris Agglutinin (LCA), Fluorescein, 5 mg
Artikelnummer: VEC-FL-1041
Hersteller Artikelnummer: FL-1041
Alternativnummer: VEC-FL-1041
Hersteller: Vector Laboratories
Kategorie: Sonstiges
Lens Culinaris Agglutinin (LCA), Fluorescein, 5 mg

Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Fluorescein labeled LCA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

 

Specifications

 
Unit Size 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Mannose, Glucose