Peanut Agglutinin (PNA), Fluorescein, 5 mg

Artikelnummer: VEC-FL-1071-5
Artikelname: Peanut Agglutinin (PNA), Fluorescein, 5 mg
Artikelnummer: VEC-FL-1071-5
Hersteller Artikelnummer: FL-1071-5
Alternativnummer: VEC-FL-1071-5
Hersteller: Vector Laboratories
Kategorie: Sonstiges
Peanut Agglutinin (PNA), Fluorescein, 5 mg

Description

Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

Fluorescein labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

 

Specifications

 
Unit Size 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Inhibiting and/or Eluting Sugar 200 mM galactose (S-9003)
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01mM MnCl2
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Galactose