Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein, 2 mg
Artikelnummer:
VEC-FL-1211
Hersteller Artikelnummer:
FL-1211
Alternativnummer:
VEC-FL-1211
Hersteller:
Vector Laboratories
Kategorie:
Sonstiges
Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein, 2 mg
Description
This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.
Fluorescein labeled GSL II has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Specifications
Unit Size
2 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage
2-8°C
Maximum Excitation
495-500 nm
Maximum Emission
514-521 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 5mM N-Acetylglucosamine
Concentration
2 mg active conjugate/ml
Conjugate
Fluorescein
Color of Fluorescence
Green
Sugar Specificity
N-Acetylglucosamine
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