Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein, 2 mg

Artikelnummer: VEC-FL-1211
Artikelname: Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein, 2 mg
Artikelnummer: VEC-FL-1211
Hersteller Artikelnummer: FL-1211
Alternativnummer: VEC-FL-1211
Hersteller: Vector Laboratories
Kategorie: Sonstiges
Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein, 2 mg

Description

This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. 

Fluorescein labeled GSL II has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

 

Specifications

 
Unit Size 2 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8°C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 5mM N-Acetylglucosamine
Concentration 2 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylglucosamine