Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
Highly purified and possess very low non-specific binding properties
Extremely high affinity for biotin
Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
Excitation at 592 nm
Emission at 617 nm
Color: Red
Specifications
Unit Size
1 mg
Applications
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage
Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation
592 nm
Maximum Emission
617 nm
Recommended Storage
2-8 °C
Concentration
1.0 mg/ml active conjugate
Conjugate
DyLight 594
Color of Fluorescence
Red
Format
Concentrate
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
