DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine), 1 Kit

Artikelnummer: VEC-SK-4100
Artikelname: DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine), 1 Kit
Artikelnummer: VEC-SK-4100
Hersteller Artikelnummer: SK-4100
Alternativnummer: VEC-SK-4100
Hersteller: Vector Laboratories
Kategorie: Kits/Assays
DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3-diaminobenzidine), 1 Kit

Description

This kit contains all of the reagents necessary to prepare either a DAB or a DAB/nickel substrate working solution. Vector DAB Substrate (3,3’-diaminobenzidine) produces a brown reaction product in the presence of peroxidase (HRP) enzyme. This kit also includes nickel chloride, which, when added to the substrate working solution, results in a gray-black reaction product. Vector DAB Substrate is suitable for darkfield and electron microscopy (EM).

 

Features of this kit:

  • Ideal for IHC/ICC/ISH and blots
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

 

Specifications

 
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Brown, Gray-Black
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Non-Aqueous (Permanent)

 

Kit Components

What's in the box:

  • 6 ml DAB Reagent 1
  • 6 ml DAB Reagent 2
  • 6 ml DAB Reagent 3
  • 6 ml DAB Reagent 4 (Nickel Optional)