Peroxidase conjugated anti-biotin antibodies are produced with the highest specific acitivity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody is chosen to provide stable, highly active conjugates for immunohistochemical, in situ hybridization, enzyme immunoassay, or transfer blot applications.
10 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 0.05% thimerosal
Recommended Usage
Moles peroxidase/mole IgG:3.3 Recommended diluent for microtitration plate assays: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP anti-biotin is 0.5 - 5.0 µg/ml. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase.
Detection Target
Biotin
Conjugate
Peroxidase
Host Species
Goat
Format
Concentrate
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