Quick-DNA/RNA Viral MagBead kit is designed for high-throughput purification of viral DNA and/or RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies and swab and fecal samples stored in DNA/RNA Shield (for sample collection, nucleic acid preservation and inactivation of pathogens).
The kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. Small (> 50 nt) and large (> 200 kb) DNA and RNA are bound to magnetic beads, washed and eluted.
The isolated high-quality nucleic acids are ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/qPCR detection.
HIGHLIGHTS
- High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal and biopsy samples
- High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
- DNA/RNA Shield included for sample collection, inactivation and storage.
Technical Specifications
Binding Capacity |
10 µg DNA/RNA per 20 µl MagBinding Beads. |
Elution Volume |
≥50 µl DNase/RNase-Free Water. |
Equipment Needed |
Magnetic stand or separator, liquid handler or robotic sample processor (user provided). |
Materials |
(avaliable separately):
ZR-96 MagStand (P1005)
Collection Plate (C2002; capacity 1.2 ml/well)
96-Well Block (P1001; capacity 2 ml/well)
Elution Plate (C2003; capacity 0.35 ml/well)
Cover Foil (C2007; 2, 6, 12 or 24 pack) |
Purity |
High-quality DNA/RNA ready for Next-Gen sequencing, RT/PCR, hybridization, etc. |
Sample Type |
≤ 400 µl plasma, serum, urine, cell culture media, blood, saliva, cellular suspension, swab, fecal sample or ≤ 5 mg biopsy sample. |
Size |
50 nt to ~200 kb |
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