Human-IgG-Neutralizing Monoclonal Antibodies Block the SARS-CoV-2 Infection

Cell Reports, 2020

A team of scientists, including researchers from Active Motif China, developed recombinant antibodies targeting SARS-CoV-2 and their work was recently published in the journal Cell Reports.

Several of these antibodies were strong binders to the SARS-CoV-2 Spike S1 protein and were able to effectively neutralize infectivity of the virus, suggesting that they could be further developed and used as therapeutics against COVID-19. Learn in the following article how Wan et al. identified 11 potent neutralizingantibodies against COVID-19 from 11convalescent patients targeting threemain epitopes on RBD.

 

Summary

Coronavirus disease 2019 (COVID-19) has become a worldwide threat to humans, and neutralizing antibodies have therapeutic potential. We have purified more than 1,000 memory B cells specific to SARS-CoV-2 S1 or its RBD (receptor binding domain) and obtain 729 paired heavy- and light-chain fragments. Among these, 178 antibodies test positive for antigen binding, and the majority of the top 17 binders with EC50 below 1 nM are RBD binders. Furthermore, we identify 11 neutralizing antibodies, eight of which show IC50 within 10 nM, and the best one, 414-1, with IC50 of 1.75 nM. Through epitope mapping, we find three main epitopes in RBD recognized by these antibodies, and epitope-B antibody 553-15 could substantially enhance the neutralizing abilities of most of the other antibodies. We also find that 515-5 could cross neutralize the SARS-CoV pseudovirus. Altogether, our study provides 11 potent human neutralizing antibodies for COVID-19 as therapeutic candidates.

SARS-CoV-2 Spike Antibodies used in this study are available through Biozol:

Product name	Clone	Product code
SARS-CoV-2 Spike Antibody (AM009105)	105-9	ACM-91337
SARS-CoV-2 Spike Antibody (AM038105)	105-38	ACM-91339
SARS-CoV-2 Spike Antibody (AM043105)	105-43	ACM-91341
SARS-CoV-2 Spike Antibody (AM004414)	414-4	ACM-91345
SARS-CoV-2 Spike Antibody (AM005415)	415-5	ACM-91347
SARS-CoV-2 Spike Antibody (AM002414)	414-2	ACM-91349
SARS-CoV-2 Spike Antibody (AM006415)	415-6	ACM-91351
SARS-CoV-2 Spike Antibody (AM032414)	414-3-2	ACM-91359
SARS-CoV-2 Spike Antibody (AM001414)	414-1	ACM-91361
SARS-CoV-2 Spike Antibody (AM015553)	553-15	ACM-91377
SARS-CoV-2 Spike Antibody (AM002413)	413-2	ACM-91379
SARS-CoV-2 Spike Antibody (AM020553)	553-20	ACM-91381
SARS-CoV-2 Spike Antibody (AM005505)	505-5	ACM-91383
SARS-CoV-2 Spike IgG/IgM Antibody (AM043105)	105-43	ACM-91385
SARS-CoV-2 Spike Antibody (AM017553)	553-17	ACM-91387