30.07.2020

Human-IgG-Neutralizing Monoclonal Antibodies Block the SARS-CoV-2 Infe... Cell Reports, 2020

A team of scientists, including researchers from Active Motif China, developed recombinant antibodies targeting SARS-CoV-2 and their work was recently published in the journal Cell Reports. Several of these antibodies were strong binders to the SARS-CoV-2 Spike S1 protein and were able to effectively neutralize infectivity of the virus, suggesting that they could be further developed and used as therapeutics against COVID-19. Learn in the following article how Wan et al. identified 11 potent neutralizingantibodies against COVID-19 from 11con...

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23.07.2020

Immunological and inflammatory profiles in mild and severe cases of CO... Nat Commun 11, 2020

Our partner AimPlex leverages years of assay development expertise to offer comprehensive solutions in biomedical testing for multiplexed bead-based immunoassays. Founded in August 2011, the company is committed to developing and providing high-quality, cost-effective assay reagents for flow cytometry analyses. AimPlex immunoassay products employ existing flow cytometry analytical platforms for high-sensitivity detection. In the following work, Song et al. were for the first time able to study the immunological and inflammatory profiles of COVID-19 pa...

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11.07.2020

Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous... PLoS One, 2019

Our partner AimPlex leverages years of assay development expertise to offer comprehensive solutions in biomedical testing for multiplexed bead-based immunoassays. Founded in August 2011, the company is committed to developing and providing high-quality, cost-effective assay reagents for flow cytometry analyses. AimPlex immunoassay products employ existing flow cytometry analytical platforms for high-sensitivity detection. In the following nothewor...

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29.06.2020

Prostaglandin F2α influences pre-ovulatory follicle characteristics an... Theriogenology, 2020

Highlights Treatment of anovular cows with a second dose of 25 mg of prostaglandin F2α increased pregnancy per AI in normothermic cows. Treatment with prostaglandin F2α had minor impacts on LH pulsatility, but increased the size and estradiol content in the pre-ovulatory follicle. Prostaglandin has a role in fertility of anovular cows that is unrelated to its luteolytic effect. In a recent

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09.06.2020

Heightened Innate Immune Responses in theRespiratory Tract of COVID-19... Cell Host & Microbe

SUMMARY The outbreaks of 2019 novel coronavirus disease (COVID-19) caused by SARS-CoV-2 infection have posed asevere threat to global public health. It is unclear how the human immune system responds to this infection.Here, we used metatranscriptomic sequencing to profile immune signatures in the bronchoalveolar lavage fluidof eight COVID-19 cases. The expression of proinflammatory genes, especially chemokines, was markedlyelevated in COVID-19 cases compared to community-acquired pneumonia patients and healthy controls, sug-gesting that SARS...

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28.05.2020

A CRISPR-Cas9-based reporter system forsingle-cell detection of extrac... Nature Communications, 2020

Abstract Extracellular vesicles (EVs) form an endogenous transport system for intercellular transfer ofbiological cargo, including RNA, that plays a pivotal role in physiological and pathologicalprocesses. Unfortunately, whereas biological effects of EV-mediated RNA transfer areabundantly studied, regulatory pathways and mechanisms remain poorly defined due to alack of suitable readout systems. Here, we describe a highly-sensitive CRISPR-Cas9-basedreporter system that allows direct functional study of EV-mediated transfer of...

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05.05.2020

Porcine Hemagglutinating Encephalomyelitis Virus Activation of the Int... Journal of Virology, 2019

PHEV, a member of the Coronaviridae family, is a typical neurotropic virus that primarily affects the nervous system of piglets to produce typical neurological symptoms. However, the mechanism of nerve damage caused by the virus has not been fully elucidated. Actin is an important component of the cytoskeleton of eukaryotic cells and serves as the first obstacle to the entry of pathogens into host cells. Additionally, the morphological structure and function of nerve cells depend on the dynamic regulation of the actin skeleton. Therefore, exp...

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27.04.2020

SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronch... The EMBO Journal, 2020

The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived fr...

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20.04.2020

A bioorthogonal system reveals antitumour immune function of pyroptosi... Nature, volume 579

Abstract Bioorthogonal chemistry capable of operating in live animals is needed to investigate biological processes such as cell death and immunity. Recent studies have identified a gasdermin family of pore-forming proteins that executes inflammasome-dependent and -independent pyroptosis1,2,3,

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01.04.2020

SARS-CoV-2 Cell Entry Is Blocked by a Clinically Proven Protease Inhib... CELL, 2020

As described in their recent Cell study, Hoffmann et al. identified the serine protease TMPRSS2 as a critical factor in the priming of the SARS-CoV-2 spike protein with its receptor ACE2, and demonstrated that a clinically proven protease inhibitor, camostat mesylate, is able to interfere with SARS-CoV-2 infection of lung cells. Featured Products: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) SARS-CoV / SARS-CoV-2 (COV...

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